MicroRNA Profiling of Fresh Lung Adenocarcinoma and Adjacent Normal Tissues from Ten Korean Patients Using miRNA-Seq

MicroRNA transcriptomes from fresh tumors and the adjacent normal tissues were profiled in 10 Korean patients diagnosed with lung adenocarcinoma using a next-generation sequencing (NGS) technique called miRNA-seq. The quality was assessed FastQC, low-quality or adapter-contaminated portions of reads removed Trim Galore. Quality-assured analyzed miRDeep2 Bowtie. abundance known miRNAs estimated per million (RPM) normalization method. Subsequently, DESeq2 Wx, we identified differentially expressed potential miRNA biomarkers for compared to tissues, respectively. We defined reliable as those detected by both methods. miRNA-seq data are available Gene Expression Omnibus (GEO) database under accession number GSE196633, all processed can be accessed via Mendeley website.